DNA Polymerases
Agilent Technologies PFUTURBO DNA POLYMERASE
PfuTurbo DNA Polymerase 1000 U Robust high-fidelity enzyme for PCR cloning with enhanced PCR product yield and length capabilities Contains: PfuTurbo DNA polymerase 10X cloned Pfu buf
New England Biolabs, Inc. LongAmp Taq 2X Master Mix – 500 reactions (50 µl vol)
The LongAmp Taq 2X Master Mix combines high quality NEB recombinant Taq DNA Polymerase with a trace amount of Deep Vent DNA Polymerase. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. This ready-to-use mix offers two fold higher fidelity than Taq DNA Polymerase alone. A wide range of PCR products can be generated, up to 30 kb from lambda or human genomic DNA.
New England Biolabs, Inc. LongAmp Taq 2X Master Mix – 100 reactions (50 µl vol)
The LongAmp Taq 2X Master Mix combines high quality NEB recombinant Taq DNA Polymerase with a trace amount of Deep Vent DNA Polymerase. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. This ready-to-use mix offers two fold higher fidelity than Taq DNA Polymerase alone. A wide range of PCR products can be generated, up to 30 kb from lambda or human genomic DNA.
American Research Products Inc Human RNA polymerase III Pol
Human RNA polymerase III (Pol III) antibody ELISA kit from CUSABIO Detection Range Sensitivity Sample serum Method Indirect
New England Biolabs, Inc. BaeGI – 500 units
A restriction endonuclease that recognizes the sequence G_KGCM^C.
Roche Diagnostics KAPA2G ROBUST DNA POLYMERASE W
KAPA2G ROBUST DNA POLYMERASE W *This item is non returnable.
Roche Diagnostics KAPA TAQ DNA POLYMERASE W/O D
KAPA TAQ DNA POLYMERASE W/O D *This item is non returnable.
Sigma Aldrich Fine Chemicals Biosciences Taq DNA Polymerase 1 U/mul
The enzyme was cloned in E.coli and is isolated to be free of unspecific endo- or exonucleases according to the current quality control procedurese. Taq DNA Polymerase is a highly processive 5primendash3prime DNA polymerase that lacks 3primendash5prime exonuclease activity. It consists of a single polypeptide chain with a molecular weight of approximately 95 kDa. The enzyme exhibits highest activity at a pH of around 9 (adjusted at 20 C) and temperatures around 75 C. Taq DNA Polymerase also accepts modified deoxyribonucleosidetriphosphates as substrates and can be used to label DNA-fragments either with radionucleotides digoxigenin fluorescein or biotin.The high processivity absence of exonuclease activity and temperature optima of Taq DNA Polymerase enable the use of this enzyme in DNA sequencing especially where the reSOLUTIONon of secondary structures plays a major role.
New England Biolabs, Inc. T7 DNA Polymerase (unmodified) – 1500 units
T7 DNA Polymerase catalyzes the replication of T7 phage DNA during infection. The protein dimer has two catalytic activities: DNA polymerase activity and strong 3 5 exonuclease. The high fidelity and rapid extension rate of the enzyme make it particularly useful in copying long stretches of DNA template.
New England Biolabs, Inc. Taq DNA Polymerase with Standard Taq (Mg-free) Buffer – 400 units
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. It is supplied with 10X Standard Taq (Mg-free) Reaction Buffer and MgCl2. 10X Standard Taq (Mg-free) Reaction Buffer is detergent-free and designed to be compatible with existing assay systems.
New England Biolabs, Inc. T7 DNA Polymerase (unmodified) – 300 units
T7 DNA Polymerase catalyzes the replication of T7 phage DNA during infection. The protein dimer has two catalytic activities: DNA polymerase activity and strong 3 5 exonuclease. The high fidelity and rapid extension rate of the enzyme make it particularly useful in copying long stretches of DNA template.
Sigma Aldrich Fine Chemicals Biosciences Pwo DNA Polymerase
Pwo DNA Polymerase is a highly processive thermostable 5AE-3AE DNA polymerase and possesses a 3AE-5AE exonuclease activity also known as proofreading activity. The enzyme has no detectable 5AE-3AE exonuclease activity. Pwo DNA Polymerase exhibits increased thermal stability with a half-life of more than two hours at 100 C compared to Taq DNA Polymerase half-life of less than 5 minutes at this temperature. Pwo DNA Polymerase-generated PCR products are blunt-ended and can therefore be used directly for blunt-end ligation without any pretreatment of the ends.
Sigma Aldrich Fine Chemicals Biosciences MTPTM Taq DNA Polymerase T
MTP Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme has both 5-3 DNA polymerase and exonuclease activities is 95 kDa by SDS-PAGE and has no detectable endonuclease or 3-5 exonuclease activities. Each lot of MTP Taq undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA.Contaminating DNA present in most other polymerase preparations often preclude or obscure the accurate interpretation of results especially when targeting conserved sequences e.g. bacterial 16S rRNA region. Through Sigmas proprietary DNA removal methods and strict quality control standards we can ensure the absence of the most commonly found contaminant DNA. Each lot of MTP Taq is assayed using PCR and primers specific to (1) the conserved region of bacterial 16S rRNA (2) the Taq expression vector and (3) the human bet